유전체학 실험분석 지노믹웍스

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Gene expression Microarray

GenomicWorks에서 제공하는 Expression Microarray 서비스

  • Agilent, Affymetrix, Nimblegen 제조사의 제품군
  • Total RNA isolation & Quality Control
  • Whole genome gene expression microarray
  • Model organism gene expression microarray
  • Customized gene expression microarray
  • Data analysis & 방문 discussion

Sample QC

Experimental Workflow

Gene Expression Microarray - 실험 진행 과정

Sample requirements

concentration Concentration (ng/ml) Quality
Total RNA 2 ~ 3 ㎍(in general) 100 ~ 200 OD(260/280)>1.8, OD(260/230)>2.0,
28s/18s > 1.5 and RIN>7.0
Cell, tissues Inquiry Inquiry .

Bioinformatic analysis - contents

분석 단계 contents
1차 분석 1. Microarray Scanning
2. Determination of spots (probes) signal intensity
3. Quantification (raw data)
4. Normalization (global (LOWESS) normalization, specific normalization)
2차 분석 1. Data filtering (Flag-out or filtering low signal intensity probes)
2. Differentially expression gene (DEG) screening (fold-change analysis or statistic analysis)
3. Common genes (Venn diagram) analysis
4. Gene ontology analysis
5. Pathway analysis
3차 분석 Customized data analysis