H > 서비스 > DNA Microarray >Gene expression Microarray
Gene expression Microarray
GenomicWorks에서 제공하는 Expression Microarray 서비스
- Agilent, Affymetrix, Nimblegen 제조사의 제품군
- Total RNA isolation & Quality Control
- Whole genome gene expression microarray
- Model organism gene expression microarray
- Customized gene expression microarray
- Data analysis & 방문 discussion
Sample QC
Experimental Workflow
Gene Expression Microarray - 실험 진행 과정
Sample requirements
| concentration | Concentration (ng/ml) | Quality | |
|---|---|---|---|
| Total RNA | 2 ~ 3 ㎍(in general) | 100 ~ 200 | OD(260/280)>1.8, OD(260/230)>2.0, 28s/18s > 1.5 and RIN>7.0 |
| Cell, tissues | Inquiry | Inquiry | . |
Bioinformatic analysis - contents
| 분석 단계 | contents |
|---|---|
| 1차 분석 | 1. Microarray Scanning 2. Determination of spots (probes) signal intensity 3. Quantification (raw data) 4. Normalization (global (LOWESS) normalization, specific normalization) |
| 2차 분석 | 1. Data filtering (Flag-out or filtering low signal intensity probes) 2. Differentially expression gene (DEG) screening (fold-change analysis or statistic analysis) 3. Common genes (Venn diagram) analysis 4. Gene ontology analysis 5. Pathway analysis |
| 3차 분석 | Customized data analysis |
