H > 서비스 > DNA Microarray >miRNA microarray
miRNA microarray
GenomicWorks에서 제공하는 miRNA Microarray 서비스
- Agilent miRNA microarray의 제품군
- Total RNA isolation & Quality Control
- Human, Mouse, Rat miRNA microarray
- Customized miRNA microarray
- Data analysis & 방문 discussion
실험 원리
Sample QC
Experimental Workflow
Agilent miRNA Microarray - 실험 진행 과정
Sample requirements
| concentration | Concentration (ng/ml) | Quality | |
|---|---|---|---|
| Total RNA | >500 ng (in general) | >50 ~ 100 | OD(260/280)>1.8, OD(260/230)>2.0, 28s/18s > 1.5 and RIN>7.0 |
| Cell, tissues | Inquiry | Inquiry |
Bioinformatic analysis - contents
| 분석 단계 | contents |
|---|---|
| 1차 분석 | 1. Microarray Scanning 2. Determination of spots (probes) signal intensity 3. Quantification (raw data) 4. Normalization |
| 2차 분석 | 1. Data filtering (Flag-out or filtering low signal intensity probes) 2. Differentially expression gene (DEG) screening (fold-change analysis or statistic analysis) 3. miRNA target genes prediction (Sanger DB) |
| 3차 분석 | Customized data analysis |
