유전체학 실험분석 지노믹웍스

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miRNA microarray

GenomicWorks에서 제공하는 miRNA Microarray 서비스

  • Agilent miRNA microarray의 제품군
  • Total RNA isolation & Quality Control
  • Human, Mouse, Rat miRNA microarray
  • Customized miRNA microarray
  • Data analysis & 방문 discussion

실험 원리

Sample QC

Experimental Workflow

Agilent miRNA Microarray - 실험 진행 과정

Sample requirements

concentration Concentration (ng/ml) Quality
Total RNA >500 ng (in general) >50 ~ 100 OD(260/280)>1.8, OD(260/230)>2.0,
28s/18s > 1.5 and RIN>7.0
Cell, tissues Inquiry Inquiry

Bioinformatic analysis - contents

분석 단계 contents
1차 분석 1. Microarray Scanning
2. Determination of spots (probes) signal intensity
3. Quantification (raw data)
4. Normalization
2차 분석 1. Data filtering (Flag-out or filtering low signal intensity probes)
2. Differentially expression gene (DEG) screening (fold-change analysis or statistic analysis)
3. miRNA target genes prediction (Sanger DB)
3차 분석 Customized data analysis