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CpG methylaton microarray

GenomicWorks¿¡¼­ Á¦°øÇÏ´Â CpG methylation Microarray ¼­ºñ

  • Agilent, Affymetrix, Nimblegen Á¦Á¶»çÀÇ Á¦Ç°±º
  • genomic DNA isolation & Quality Control
  • Methylated DNA enrichment (MeDIP)
  • Human, Mouse, Rat CpG methylation microarray
  • Model organism CpG methylation microarray
  • Data analysis & ¹æ¹® discussion

MeDIP

Experimental Workflow

CpG Methylation Microarray - ½ÇÇè ÁøÇà °úÁ¤

Sample requirements

concentration Concentration (ng/ml) Quality
Genomic DNA > 10§¶ (in general) > 100 ~ 200 OD(260/280)>1.8 is highly recommended
Cell, tissues Inquiry Inquiry

Bioinformatic analysis - contents

ºÐ¼® ´Ü°è contents
1Â÷ ºÐ¼® 1. Microarray Scanning
2. Determination of spots (probes) signal intensity
3. Quantification (raw data)
4. Normalization (global (LOWESS) normalization, specific normalization)
2Â÷ ºÐ¼® 1. Data filtering (Flag-out or filtering low signal intensity probes)
2. Differentially methylated probes screening (fold-change analysis or statistic analysis)
3. Hierarchical clustering
3Â÷ ºÐ¼® Customized data analysis