유전체학 실험분석 지노믹웍스

H > 서비스 > DNA Microarray > CpG methylaton microarray

CpG methylaton microarray

GenomicWorks에서 제공하는 CpG methylation Microarray 서비

  • Agilent, Affymetrix, Nimblegen 제조사의 제품군
  • genomic DNA isolation & Quality Control
  • Methylated DNA enrichment (MeDIP)
  • Human, Mouse, Rat CpG methylation microarray
  • Model organism CpG methylation microarray
  • Data analysis & 방문 discussion

MeDIP

Experimental Workflow

CpG Methylation Microarray - 실험 진행 과정

Sample requirements

concentration Concentration (ng/ml) Quality
Genomic DNA > 10㎍ (in general) > 100 ~ 200 OD(260/280)>1.8 is highly recommended
Cell, tissues Inquiry Inquiry

Bioinformatic analysis - contents

분석 단계 contents
1차 분석 1. Microarray Scanning
2. Determination of spots (probes) signal intensity
3. Quantification (raw data)
4. Normalization (global (LOWESS) normalization, specific normalization)
2차 분석 1. Data filtering (Flag-out or filtering low signal intensity probes)
2. Differentially methylated probes screening (fold-change analysis or statistic analysis)
3. Hierarchical clustering
3차 분석 Customized data analysis