H > ¼ºñ½º > DNA Microarray > CpG methylaton microarray
CpG methylaton microarray
GenomicWorks¿¡¼ Á¦°øÇÏ´Â CpG methylation Microarray ¼ºñ
- Agilent, Affymetrix, Nimblegen Á¦Á¶»çÀÇ Á¦Ç°±º
- genomic DNA isolation & Quality Control
- Methylated DNA enrichment (MeDIP)
- Human, Mouse, Rat CpG methylation microarray
- Model organism CpG methylation microarray
- Data analysis & ¹æ¹® discussion
MeDIP
Experimental Workflow
CpG Methylation Microarray - ½ÇÇè ÁøÇà °úÁ¤
Sample requirements
concentration | Concentration (ng/ml) | Quality | |
---|---|---|---|
Genomic DNA | > 10§¶ (in general) | > 100 ~ 200 | OD(260/280)>1.8 is highly recommended |
Cell, tissues | Inquiry | Inquiry |
Bioinformatic analysis - contents
ºÐ¼® ´Ü°è | contents |
---|---|
1Â÷ ºÐ¼® | 1. Microarray Scanning 2. Determination of spots (probes) signal intensity 3. Quantification (raw data) 4. Normalization (global (LOWESS) normalization, specific normalization) |
2Â÷ ºÐ¼® | 1. Data filtering (Flag-out or filtering low signal intensity probes) 2. Differentially methylated probes screening (fold-change analysis or statistic analysis) 3. Hierarchical clustering |
3Â÷ ºÐ¼® | Customized data analysis |