H > 서비스 > DNA Microarray > CpG methylaton microarray
CpG methylaton microarray
GenomicWorks에서 제공하는 CpG methylation Microarray 서비
- Agilent, Affymetrix, Nimblegen 제조사의 제품군
- genomic DNA isolation & Quality Control
- Methylated DNA enrichment (MeDIP)
- Human, Mouse, Rat CpG methylation microarray
- Model organism CpG methylation microarray
- Data analysis & 방문 discussion
MeDIP
Experimental Workflow
CpG Methylation Microarray - 실험 진행 과정
Sample requirements
| concentration | Concentration (ng/ml) | Quality | |
|---|---|---|---|
| Genomic DNA | > 10㎍ (in general) | > 100 ~ 200 | OD(260/280)>1.8 is highly recommended |
| Cell, tissues | Inquiry | Inquiry |
Bioinformatic analysis - contents
| 분석 단계 | contents |
|---|---|
| 1차 분석 | 1. Microarray Scanning 2. Determination of spots (probes) signal intensity 3. Quantification (raw data) 4. Normalization (global (LOWESS) normalization, specific normalization) |
| 2차 분석 | 1. Data filtering (Flag-out or filtering low signal intensity probes) 2. Differentially methylated probes screening (fold-change analysis or statistic analysis) 3. Hierarchical clustering |
| 3차 분석 | Customized data analysis |
